Abortive Infection of Shigella dysenteriae P2 by T2 Bacteriophage

Abortive infection of Shigella dysenteriae P2 by T2 bacteriophage.

We have investigated some of the biochemical events that accompany the abortive infection by T2 of Shigella dysenteriae lysogenized with the temperate phage P2. After infection with T2, protein and RNA synthesis continued for 3 to 5 min. The virus-induced enzyme, deoxycytidylate hydroxymethylase was produced in reduced amounts (15% of normal), and the extent of deoxyribonucleic acid (DNA) synth...

Separation of deoxyribo-oligonucleotidases induced by infection with bacteriophage T2.

In 1962, Aposhian and Kornberg' described a procedure for partial purification of the DNA polymerase induced by infection of Escherichia coli with bacteriophage T2. They observed that their purest preparation of phage-induced DNA polymerase exhibited deoxyribonuclease activity when assayed with heat-denatured DNA as substrate. Recently, Lucas and Kornberg2,3 purified the DNA polymerase induced ...

A Deoxyribonuclease Induced by Infection with Bacteriophage T2.

The Multiplication of Bacteriophage in Vivo and Its Protective Effect against an Experimental Infection with Shigella Dysenteriae

1. Anti-Shiga bacteriophage injected into the general circulation can multiply in the brain of mice infected intracerebrally with Shigella dysenteriae. 2. Under proper conditions, the injection of active bacteriophage into the general circulation can protect mice against an otherwise fatal intracerebral infection with Shigella dysenteriae. 3. The protection so induced appears to depend upon the...

Survival of Shigella dysenteriae on fomites

Studies have shown that various objects, such as utensils, toys, and clothes, can serve as vehicles for transmission of Shigella spp. Shigellae can become viable but non-culturable (VBNC) when exposed to various environmental conditions as shown in earlier studies. The present study was carried out to detect VBNC Shigella dysenteriae type 1 on various fomites by direct viable counting, polymera...